Recombinant Mouse RSPO1 Protein, His Tag
产品名称: Recombinant Mouse RSPO1 Protein, His Tag
英文名称: Recombinant Mouse RSPO1 Protein, His Tag
产品编号: 92277ES08
产品价格: 685.00
产品产地: Yeasen
品牌商标: Yeasen
更新时间: 2024-12-10T11:18:52
使用范围: null
规格 | 价格 |
5μg | 685.0 |
50μg | 2985.0 |
1 mg | 31425.0 |
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产品信息
产品名称 |
产品编号 |
规格 |
Recombinant Mouse RSPO1 Protein |
92277ES08 |
5 μg |
92277ES50 |
50 μg |
|
92277ES80 |
1mg |
产品简介
R-Spondin 1 (RSPO1, Roof plate-specific Spondin 1), also known as cysteine-rich and single thrombospondin domain containing protein 3. Interest in R-Spondin 1 as a cell culture supplement has grown with the expansion of the organoid field. R-Spondin 1 is widely used in organoid cell culture workflows as a vital component that promotes both growth and survival of 3D organoids .
性能参数
Synonyms |
R-Spondin 1;Roof Plate-specific Spondin 1;Cristin3; R-spondin-1 |
Source |
Expressed in E. coli. |
Molecular Weight |
Approximately 20.9kDa, a single non-glycosylated polypeptide chain containing 189 amino acid |
Accession |
Q9Z132: Ser21-Gly209 |
Biological Activity |
Fully biologically active when compared to standard. Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The typical ED50 is 50-200 ng/mL in the presence of 5ng/mL recombinant mouse Wnt-3a. |
Purity |
> 96% by SDS-PAGE. |
Endotoxin |
<0.01EU/μg protein as determined by LAL method. |
Formulation |
Lyophilized from a 0.2mm filtered solution in 20mM PB, 150mM NaCl, pH 7.4. |
Reconstitution |
Before use this product, please read the direction below carefully. 1.This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. 2.Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
储存条件
The product should be stored at -25~-15℃ for 1 year from date of receipt.
1 month, 2 ~8 °C under sterile conditions after reconstitution.
3 months, -25~-15℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
注意事项
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.
Ver. CN20231116