超低分子量蛋白marker-null-资讯-生物在线

超低分子量蛋白marker

作者:盛世中方(北京)生物科技有限公司 2008-05-31T00:00 (访问量:8391)

Product Description
The Color Marker Ultra Low Range is designed for use
in a Tris-tricine SDS-PAGE system and in transfer to
solid phase supports such as nitrocellulose, nylon, and
PVDF membranes. The Color Marker Ultra Low Range
consists of six peptides and proteins, each conjugated
to a colored dye, thereby, providing a visual monitor of
migration during electrophoresis and/or a visual check
of transfer efficiency to a membrane.
Each vial of marker contains 200 ml of a mixture of six
dye conjugates (see Table 1) in 10 mM Tris-HCl,
pH 7.0, containing 0.5% SDS, 2 mM EDTA, 10 mM
dithiothreitol, 33% glycerol, 4 M urea, and 0.01%
sodium azide.
When precise molecular mass determinations are
required, use the Ultra Low Range Molecular Weight
Marker, Catalog Number M3546.
The Color Marker Ultra Low Range may be stained with
Brilliant Blue G, but band intensity will vary. The marker
produces relatively broad bands when compared to
unconjugated proteins.

 

 

Polypeptide

Native Molecular

Mass of Subunit

(Daltons)*

                          Color of   Conjugate

Triosephosphate

Isomerase,

rabbit muscle

 

26,600

 

Orange

Myoglobin,

horse heart

 17,000

Violet

a-Lactalbumin,

bovine milk

 14,200

Red

Aprotinin, bovine lung

 6,500

Blue

Insulin Chain B,

Oxidized, bovine

 3,496

Blue

Bradykinin

 1,060

Blue

 

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